Pediatric Vitamin D Status: Age, Gender, and Seasonal Determinants in a Comprehensive Retrospective Cohort

Authors

Ahmet Dündar, Songül Çetik Yıldız, Halit Irmak
https://doi.org/10.18621/eurj.1783317
Objectives: Vitamin D plays a critical role in bone health, immune function, and overall growth and development in children. So, the study aimed to determine serum vitamin D levels in a large sample, examine their relationship to age groups, gender, seasonal and monthly, and identify risk groups by demonstrating the prevalence of vitamin D deficiency in the pediatric age group.
Methods: A retrospective study was conducted using serum vitamin D level data from pediatric patients presenting to the Pediatrics Outpatient Clinic. Children were divided into four age groups based on developmental stages: 1-4, 5-8, 9-12, and 13-17 years. Vitamin D levels were categorized as severe deficiency, deficiency, insufficiency, and normal. The data were analyzed for age, gender, seasonality, and monthly distribution, and the relationships between these variables were evaluated using comprehensive statistical methods.
Results: Only 6.5% of the average vitamin D levels were found to be normal. Deficiency, insufficiency, or severe deficiency was detected in 93.5%. A weak but significant negative correlation was observed between age and vitamin D levels. While levels were similar between genders, severe deficiency was higher in females. The highest values were observed in summer and the lowest in winter, with July-September being the peak and January-February the trough.
Conclusions: Our study revealed that vitamin D deficiency is common in children and a critical public health problem. Decreasing levels with age, seasonal cycles, and gender differences indicate that the risk becomes more pronounced. These findings highlight the need for supplementation plans and awareness-raising strategies, particularly during winter and spring.
Vitamin D Status, Pediatric Population, Deficiency, Analytical Determinants

1. Corsello A, Spolidoro GCI, Milani GP, Agostoni C. Vitamin D in pediatric age: Current evidence, recommendations, and misunderstandings. Front Med (Lausanne). 2023;10:1107855. doi: 10.3389/fmed.2023.1107855.

2. Weydert JA. Vitamin D in Children's Health. Children (Basel). 2014;1(2):208-26. doi: 10.3390/children1020208.

3. Holick MF. Vitamin D deficiency. N Engl J Med. 2007;357(3):266-281. doi: 10.1056/NEJMra070553.

4. Bagci Z. Evaluation of the Effects of Age Group, Gender and Seasonal Factors On Vitamin D Levels in 9496 Children. Selcuk Med J 2021;37(4): 365-370. doi: 10.30733/std.2021.01528.

5. Cashman KD, van den Heuvel EG, Schoemaker RJ, Prévéraud DP, Macdonald HM, Arcot J. 25-Hydroxyvitamin D as a Biomarker of Vitamin D Status and Its Modeling to Inform Strategies for Prevention of Vitamin D Deficiency within the Population. Adv Nutr. 2017;8(6):947-957. doi: 10.3945/an.117.015578.

6. Ghiga G, Țarcă E, Țarcă V, et al. Vitamin D Deficiency: Insights and Perspectives from a Five-Year Retrospective Analysis of Children from Northeastern Romania. Nutrients. 2024;16(22):3808. doi: 10.3390/nu16223808.

7. Zhou DY, Wei SM, Zhu CL, et al. Age-, season- and gender-specific reference intervals of serum 25-hydroxyvitamin D3 for healthy children (0 ~ 18 years old) in Nanning area of China. J Physiol Sci. 2024;74(1):2. doi: 10.1186/s12576-023-00895-z.

8. Sarı E, Çoban G, Çelebi ÖFZ, Açoğlu AE. The Status of Vitamin D Among Children Aged 0 to 18 Years. J Pediatr Res. 2021;10,8(4):438-443. doi: 10.4274/jpr.galenos.2021.09851

9. Yeşiltepe-Mutlu G, Aksu ED, Bereket A, Hatun Ş. Vitamin D Status Across Age Groups in Turkey: Results of 108,742 Samples from a Single Laboratory. J Clin Res Pediatr Endocrinol. 2020;12(3):248-255. doi: 10.4274/jcrpe.galenos.2019.2019.0097.

10. Isiksacan N, Bıyık I, Kasapoglu P, Koser M, Caglar FN, Kocamaz N. Increased risk of cardiovascular disease may be starting in childhood: 25OH vitamin D levels in Turkish children. J Updates Cardiovasc Med. 2018;156(1):1-6. doi: 10.15511/ejcm.18.00101.

11. Andıran N, Çelik N, Akça H, Doğan G. Vitamin D deficiency in children and adolescents. J Clin Res Pediatr Endocrinol. 2012;4(1):25-29. doi: 10.4274/jcrpe.574.

12. Zhang Y, Zhou L, Ren Y, Zhang H, Qiu W, Wang H. Assessment of serum vitamin D levels in children aged 0-17 years old in a Chinese population: a comprehensive study. Sci Rep. 2024;14(1):12562. doi: 10.1038/s41598-024-62305-7.

13. Chen Z, Lv X, Hu W, Qian X, Wu T, Zhu Y. Vitamin D Status and Its Influence on the Health of Preschool Children in Hangzhou. Front Public Health. 2021;9:675403. doi: 10.3389/fpubh.2021.675403.

14. Smyczyńska J, Smyczyńska U, Stawerska R, et al. Seasonality of vitamin D concentrations and the incidence of vitamin D deficiency in children and adolescents from central Poland. Pediatr Endocrinol Diabetes Metab. 2019;25(2):54-59. doi: 10.5114/pedm.2019.85814.

15. Davies PS, Bates CJ, Cole TJ, Prentice A, Clarke PC. Vitamin D: seasonal and regional differences in preschool children in Great Britain. Eur J Clin Nutr. 1999;53(3):195-198. doi: 10.1038/sj.ejcn.1600697.

16. Won JW, Jung SK, Jung IA, Lee Y. Seasonal Changes in Vitamin D Levels of Healthy Children in Mid-Latitude, Asian Urban Area. Pediatr Gastroenterol Hepatol Nutr. 2021;24(2):207-217. doi: 10.5223/pghn.2021.24.2.207.

17. Hansen L, Tjønneland A, Køster B, et al. Vitamin D Status and Seasonal Variation among Danish Children and Adults: A Descriptive Study. Nutrients. 2018;10(11):1801. doi: 10.3390/nu10111801.

There are 17 references in total.
1.
Dündar A, Çetik Yıldız S, Irmak H. Pediatric Vitamin D Status: Age, Gender, and Seasonal Determinants in a Comprehensive Retrospective Cohort. Eur Res J. Published online March 14, 2026:1-9. doi:10.18621/eurj.1783317

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  • Article Type Research Article
  • Submitted February 21, 2026
  • Published March 14, 2026
  • Issue 2026: Online First
  • Section Research Article
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