Online ISSN: 2149-3189
2015
Monthly
Nicaea Medical Publishing
Türkiye

Determination of minimum serum concentration to develop scaffold free micro-tissue

Ozan Karaman, Ziyşan Buse Yaralı
  • Ozan Karaman (Author) İzmir Katip Çelebi University, Tissue Engineering and Regenerative Medicine Laboratory, Department of Biomedical Engineering, İzmir https://orcid.org/0000-0002-4175-4402
  • Ziyşan Buse Yaralı (Author) İzmir Katip Çelebi University, Tissue Engineering and Regenerative Medicine Laboratory, Department of Biomedical Engineering, İzmir https://orcid.org/0000-0002-9371-6424
DOI
https://doi.org/10.18621/eurj.342109
Abstract
Objective. Formation of three-dimensional (3D) micro-tissues without scaffolds are widely used not only to define in vivo tissue formation mechanisms but also the development of different tissue-specific drugs. However, depending on high serum and growth factor concentrations, it would be hard to identify major effective biological cues on micro-tissue formation. The aim of the study is to determine the effect of different serum concentrations on Human Umbilicial Vein Endothelial Cells (HUVECs) micro-tissue formation.
Methods. Micro-tissue of HUVEC line was formed by using 3D petri dish technique with medium containing 0%, 1%, 5% and 10% fetal bovine serum (FBS). On the 7th day after micro-tissue formation, live/dead cells analysis was conducted. Micrograph taken on days 1, 3, 5 and 7th of micro-tissue formation were determined by image analysis with ImageJ.
Results. Sizes of micro-tissue formed with 0% FBS on day 1 and 3 determined as 277 ± 12 µm and 279 ± 20 µm, respectively; however, especially on day 7 micro-tissue size significantly decreased to 229 ± 6 µm. When live/dead analysis results were examined, high cell viability was observed in 5% and 10% FBS concentration. Although micro-tissue like structures were observed in 0% and 1% FBS concentrations dead cell ratio considerably increased compared to 5% and 10% FBS concentration.
Conclusions. It has been determined that 0% and 1% serum are appropriate for determining the efficacy of biomimetic peptides and different extracellular matrix proteins on micro-tissue formation parameters of HUVEC. High cell viability in micro-tissues was observed with 5% and 10% serum concentrations.
Keywords
micro-tissue, three-dimensional cell culture, serum concentration
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How to Cite
1.
Karaman O, Yaralı ZB. Determination of minimum serum concentration to develop scaffold free micro-tissue. Eur Res J. 2018;4(3):145-151. doi:10.18621/eurj.342109
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Vol. 4 No. 3 (2018)

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  • Article Type Research Article
  • Submitted February 21, 2026
  • Published July 3, 2018
  • Issue Vol. 4 No. 3 (2018)
  • Section Research Article
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